藏羚羊5个微卫星标记的多态性分析

Genetic Diversity of Endangered Tibetan Antelope Revealed by Microsatellite Markers

藏羚羊是我国青藏高原的特有物种.为分析藏羚羊独特的遗传变异特征和核DNA遗传多样性,以5个微卫星标记对50只藏羚羊的等位基因多态性进行了研究.研究结果表明：5个微卫星标记共检测到92个等位基因,每个标记的平均等位基因数为18.4个（在16~23之间）,平均有效等位基因数为11.1;5个微卫星标记的多态信息含量均在0.8573以上,为高度多态标记,其中L03标记的多态信息含量最高,达0.9372;各标记的观测杂合度在0.4898~0.9091之间,期望杂合度在0.8770~0.9504之间,平均期望杂合度为0.8990,属于高度杂合标记,遗传变异丰富.这些筛选出的多态性微卫星标记可应用于藏羚羊遗传多样性、遗传结构分析及遗传图谱的构建等工作.

Tibetan antelope（pantholops hodgsonii）,also known as the chiru,is endemic to the Qinghai-Tibet Plateau.A total of 50 individual of Tibetan antelope were analyzed using five microsatellite markers.With an average of 18.4 alleles per locus（ranging from 16 to 23）,92 alleles were identified at 5 microsatellite loci.The average effective number of allele was 11.1,and the polymorphism information content（PIC）of the 5 markers were more than 0.8573,which indicated a high polymorphism.With an value of 0.9372,the highest PIC was observed at locus L03.The observed heterozygote（Ho）of the 5 markers ranged from 0.4898 to 0.9091,and the expected heterozygote（He）varied from 0.8770 to 0.9504（average 0.8990）.The results revealed a high level of genetic variation at 5 loci,which suggested that those microsatellite markers could be applied in studies of genetic diversity,genetic constructure and construction of the genetic map in Tibetan antelope.