过氧化氢扩散和等离子体放电对嗜热脂肪杆菌芽孢杀灭效果比较

COMPARISON STUDY ON THE SPORICIDAL EFFECT OF HYDROGEN PEROXIDE GAS DIFFUSION AND PLASMA DISCHARGE ON THE Bacillus stearothermophilus SPORES

目的研究低温等离子体在灭菌过程中过氧化氢气体扩散和等离子放电杀菌效果,探讨灭菌过程的主导因素。方法采用载体定量杀菌试验方法,分别观察过氧化氢低温等离子体灭菌过程中过氧化氢扩散和等离子体放电的杀菌效果。结果在过氧化氢低温等离子体灭菌装置设定条件下,灭菌腔内温度50℃、过氧化氢扩散2.5 mg/L,不启动放电过程,作用42 s可完全杀灭载体上嗜热脂肪肝菌芽孢。在灭菌腔内温度50℃条件下,不用过氧化氢气体,仅启动等离子放电作用15 min,对载体上嗜热脂肪肝菌芽孢杀灭对数值<2.0。结论在过氧化氢低温等离子体灭菌过程中,其主导杀菌作用是气化过氧化氢及其扩散状态,等离子放电仅起到辅助作用。

Objective To compare the sterilization effect of hydrogen peroxide gas diffusion and plasma discharge during sterilization in the low - temperature plasma and explore the dominant factors in the sterilization process of low - temperature plasma. Methods Carrier quantitative germicidal test method was used to observe the sterilization effect of hydrogen peroxide gas diffusion and plasma discharge during sterilization in the low -temperature plasma. Results Under the condition of low - temperature plasma sterilization device setting, with 50℃ sterilization chamber temperature, hydrogen peroxide diffusion speed of 2.5 mg/L, no starting the discharge process and 42 s sterilization, it can completely kill the Bacillus stearothermophilus spores on the carrier. With 50℃ sterilization chamber temperature, no use of hydrogen peroxide and 15 min of plasma discharge, the logarithm is less than 2. 0 for killing the Bacillus stearothermophilus spores on the carrier. Conclusion Hydrogen peroxide and its diffusion status are the dominant factors in the sterilization process of low - temperature plasma. Plasma discharge only played a supporting role.