真空包装冷藏鲟鱼中优势腐败菌的分离、鉴定及生长预测

Isolation, Identification and Growth Prediction of Dominant Spoilage Bacteria in Cold-Stored Sturgeon under Vacuum Condition

为监测真空包装鲟鱼在冷藏过程中的优势腐败菌动态变化,通过感官评价和传统选择性培养的方法,对真空包装冷藏鲟鱼中的优势腐败菌进行了分析;同时选择性分离筛选出代表性菌株Q-1和C-1,并基于16S rRNA基因进行了鉴定。结果表明:气单胞菌和肠科菌是真空包装鲟鱼在冷藏过程中的优势腐败菌;菌株Q-1可能为维氏气单胞菌(Aeromonas veronii)新亚种,菌株C-1为路德维希肠杆菌(Enterobacter ludwigii);同时与菌株C-1相比,菌株Q-1的生长延滞期较长,但生长较快,修正的Gompertz模型能够可靠地分别预测菌株C-1和菌株Q-1在真空包装鲟鱼在冷藏过程中生长动态。

The objective of this study was to isolate and identify dominant spoilage bacteria in cold-stored sturgeon under vacuum condition, as well as make predictions of the bacteria growth. Sensory evaluation and selective culture were used to monitor the kinetic growth of dominant spoilage bacteria. Based on 16S rRNA gene sequencing, Q-1 and C-1 were identified and isolated selectively as dominant spoilage bacteria. The results showed that Aeromonas sp. and Enterobacter sp. were dominant spoilage bacteria in cold-stored sturgeon under vacuum condition. Q-1 was likely to be a new subspecies of Aeromonas veronii, and C-1 was Enterobacter ludwigii. Compared with C-1, the lag phase of Q-1 was longer and the growth rate was higher. A modified Gompertz equation could make a credible prediction of the dynamic growth of C-1 and Q-1 in the cold-stored sturgeon under vacuum condition.