摘要
Quantitative interferometric microscopy is an important method for observing biological samples such as cells and tissues. As a key step in phase recovery, a fast phase unwrapping algorithm is proposed. By shifting rood 2π wrapped phase map for one pixel, then multiplying the original phase map and the shifted one, the phase discontinuities could be easily determined with high speed and efficiency. The method aims at enhancing phase retrieving efficiency without any background knowledge. We test our algorithm with both numerical simulation and experiments, by focusing our attentions on wrapped quantitative phase maps of cells. The results indicate that this algorithm features fast, precise and reliable.
Quantitative interferometric microscopy is an important method for observing biological samples such as cells and tissues. As a key step in phase recovery, a fast phase unwrapping algorithm is proposed. By shifting rood 2π wrapped phase map for one pixel, then multiplying the original phase map and the shifted one, the phase discontinuities could be easily determined with high speed and efficiency. The method aims at enhancing phase retrieving efficiency without any background knowledge. We test our algorithm with both numerical simulation and experiments, by focusing our attentions on wrapped quantitative phase maps of cells. The results indicate that this algorithm features fast, precise and reliable.
基金
supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
the National Natural Science Foundation of China(No.31372399)
the Natural Science Foundation of Jiangsu Province(No.BK20130699)for Prof.Fei Liu