复方金银花冲剂中绿原酸含量的测定

Determination of Chlorogenic Acid Content in Compound Honeysuckle Granules

目的采用高效液相色谱法测定复方金银花冲剂中绿原酸的含量。方法色谱柱：迪马C18,250×4.6mm,5μm;柱温：30℃;流动相：乙腈-0.4%磷酸（60∶40）;流速：0.8ml/min;检测波长：327nm。结果绿原酸在0.02～0.40μg范围内与峰面积呈良好的线性关系（r=0.9995）。平均加样回收率为99.3%,RSD为0.53%（n=6）。结论该方法简便易行,结果可靠,可有效地控制复方金银花冲剂的质量。

Objective Determination of chlorogenic acid in Compound Honeysuckle Granules by HPLC. Methods Column： Dumas C18,250 × 4.6mm,5μm; column temperature： 30℃; mobile phase： acetonitrile-0. 4% phosphoric acid（ 60∶ 40）; flow rate： 0. 8ml /min; detection wavelength：327nm. Results chlorogenic acid in the range of 0. 02 ～0. 40μg peak Area showed a good linear relationship（ r =0. 9995）. The average recovery was 99. 3%,RSD was 0. 53%（ n =6）. Conclusion The method is simple,reliable,and can effectively control the quality of Compound Honeysuckle Granules.