粉防己碱对β-葡聚糖诱导的巨噬细胞生长、胞内游离钙及cAMP表达的影响

Effect of Tetrandrine on Proliferation,Expression of [Ca2＋]i and cAMP in β-glucan Stimulated RAW264.7 Cell Line

目的 观察粉防己碱（Tet）对β-葡聚糖（β-glucan）诱导的巨噬细胞增殖、胞内游离钙及环磷酸腺苷（cAMP）表达的影响.方法 以终浓度为100 μg/ml的β-glucan刺激RAW264.7细胞,建立细胞模型.MTT法检测不同浓度Tet（0,0.01,0.1,1,10,100 μmol/L）对RAW264.7细胞增殖的影响;动态检测Tet（0.01,0.1,1μmol/L）剂量组对胞内游离钙和cAMP表达的影响.结果 与对照组比较,Tet（0.01,0.1,1μmol/L）剂量组A值升高（P＜0.01）;胞内游离钙在Tet作用5 min时表达增加（t=4.18,4.17,3.40,P均＜0.01）;cAMP浓度在1 min时明显升高（t=3.33,3.84,3.18,P均＜0.01）.Tet＞10 μmol/L时,A值下降（P＜0.01）.结论 适当浓度的Tet可以促进β-glucan诱导的RAW264.7细胞生长,协同βglucan调节细胞内游离钙及cAMP的表达.

Objective To observe the effect of tetrandrine （Tet） on β glucan induced macrophage proliferation,the expression of [Ca2＋]i and cAMP.Methods With the 100 μg/ml β glucan stimulates RAW264.7 cell model.MTT to detect of Tet （0,0.01,0.1,1,10 100 μmol/L） on the proliferation of RAW264.7 cell; dynamic detection of Tet （0.01,0.1,1 μmol/L） on [Ca2＋]i and cAMP concentration.Results Compared with the control group,Tet （0.01,0.1,1 μmol/L） group A values in creased （P＜0.01） ;intracellular [Ca2＋]i expression increased in 5 min （t=4.18,P＜0.01 ; t=4.17,P＜0.01 ; t=3.40,P＜ 0.01） ; cAMP concentration increased significantly at 1 min （t=3.33,P＜0.01 ; t=3.84,P＜0.01 ; t=3.18,P＜0.01）.A values decreased significantly when Tet＞10 μmol/L （P＜0.01）.Conclusion Suitable concentration of Tet can promote the growth of β-glucan induced RAW264.7 cells,and collaborative β glucan regulates the expression of [Ca2＋] and cAMP.