多指标综合评价法优选补肾活血颗粒的醇提工艺

Optimization of Ethanol Extraction Process for Bushen Huoxue Granules by Multi-index Comprehensive Evaluation Method

目的：优选补肾活血颗粒的醇提工艺.方法：采用HPLC测定桂皮醛和苦杏仁苷含量,流动相乙腈（A）-水（B）梯度洗脱（0～3.5 min,18％A;3.5～4.0 min,18％～55％A;4.0 ～9.0 min,55％ A;9.0 ～9.5 min,55％～ 18％ A）,检测波长分别为290,210 nm.以桂皮醛、苦杏仁苷转移率为综合评价指标,通过单因素试验考察提取方法、浸泡时间、乙醇体积分数对醇提工艺的影响,利用正交试验考察提取溶剂用量、提取次数、提取时间对醇提工艺的影响.结果：最佳醇提工艺为加10倍60％乙醇浸泡1h,提取3次,每次1.5h;桂皮醛、苦杏仁苷转移率分别为85.43％,86.95％.结论：优选的提取工艺重复性好,可为补肾活血颗粒的工业生产提供参考.

Objective：To optimize ethanol extraction process of Bushen Huoxue granules.Method：HPLC was adopted to determine contents of cinnamaldehyde and amygdalin with mobile phase of acetonitrile （A）-water （B） for gradient elution （0-3.5 min,18％A; 3.5-4.0 min,18％-55％A; 4.0-9.0 min,55％A; 9.0-9.5 min,55％-18％ A）,detection wavelength at 290 and 210 nm,respectively.Taking transfer rates of cinnamaldehyde and amygdalin as a comprehensive evaluation index,effects of extracting method,soaking time and ethanol concentration on ethanol extraction process were investigated by single factor tests,orthogonal test was employed to optimize extraction process with ethanol consumption,extraction times and extracting time as factors.Result：Optimum extraction process was as follows：soaked 1 h with 10 times the amount of 60％ ethanol,extracted thrice for 1.5 h each time; transfer rates of cinnamaldehyde and amygdalin were 85.43％ and 86.95％,respectively.Conclusion：This optimized extraction technology was repeatable and feasible,it could provide a reference for industrial production of Bushen Huoxue granules.