摘要
目的:HPLC梯度洗脱法同时测定天山花楸叶提取物中的芦丁、金丝桃苷、异槲皮苷、槲皮素-3-O-(6"-O-丙二酰基)-β-D-葡萄糖苷、紫云英苷、山柰素-3-O-(6"-O-丙二酰基)-β-D-葡萄糖苷.方法:采用Waters C18色谱柱(4.6 mm × 150 mm,5 μm),乙腈-0.4%磷酸溶液为流动相梯度洗脱,运行时间30.0 min,平衡时间10 min,流速1.0 mL· min-,柱温25℃,波长360 nm.结果:芦丁、金丝桃苷、异槲皮苷、槲皮素-3-0-(6"-0-丙二酰基)-β-D-葡萄糖苷、紫云英苷、山柰素-3-0-(6"-0-丙二酰基)-β-D-葡萄糖苷分别在3.3 ~39.5,6.2 ~74.1,12.3 ~ 147.5,14.3 ~ 171.2,7.5 ~90.4,15.6~187.5 mg·L-1与峰面积呈良好的线性关系;平均加样回收率分别为98.1%,97.5%,94.5%,95.8%,102.2%,97.9%,其RSD分别为1.37%,1.57%,1.02%,1.77%,2.48%,1.38%.结论:该法操作简便、快捷、准确、稳定性及重复性好,可用于对天山花楸中6个成分的含量测定.
Objective:A high-performance liquid chromatographic method was established to evaluate the quality of Sorbus tianschanica with a simultaneous determination of six compounds [rutin,hyperoside,isoquercitrin,quercetin-3-O-(6"-O-malonyl)-β-D-glucoside,kaempferol-3-O-β-D-glucopyranoside and kaempferol-3-O-(6"-O-malonyl)-β-D-glucpyranoside].Method:The Diamonsil C18 (4.6 mm × 250 mm,5 μm) column was adopted and diluted with acetonitrile-0.4% potassium dihydrogen phosphate gradiently at the wavelength of 360 nm.The flow rate was 1.0 mL ·min-1 and the column temperature was 25 ℃.Result:The method had good linearity over the range of 3.3-39.5,6.2-74.1,12.3-147.5,14.3-171.2,7.5-90.4,15.6-187.5 mg ·L-1 for rutin,hyperoside,isoquercitrin,quercetin-3-O-(6"-O-malonyl)-β-D-glucoside,kaempferol3-O-β-D-glucopyranoside,kaempferol-3-O-(6"-O-malonyl)-β-D-glucopyranoside,respectively.The average recovery (n=6) of six components were 98.1%,97.5%,94.5%,95.8%,102.2%,and 97.9%,with RSDsof 11.37%,1.57%,1.02%,1.77%,2.48%,and 1.38% (n =6).Conclusion:The developed method is convenient,rapid and accurate with high repeatability and stability,it is suitable for the content determination.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第15期58-61,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81260627)
石河子大学国家级大学生创新创业训练计划项目(111075938)