RP-HPLC同时测定布渣叶中4种黄酮类成分

Simultaneous Determination of Four Flavonoids in Microctis Folium by HPLC

目的：建立布渣叶药材中槲皮素、山奈素、芹菜素、异鼠李素4种黄酮类成分的含量测定方法.方法：采用Waters Xbridge TM C18色谱柱（4.6 mm×250 mrn,5μm）,以甲醇-0.4％磷酸水溶液为流动相,二元梯度洗脱,流速1.0 mL·min-,检测波长367 nm,柱温35℃.结果：槲皮素、山奈素、芹菜素、异鼠李素分别在14.58 ～72.90,63.76 ～ 318.8,32.92～164.6,79.68 ～398.4 ng与峰面积呈良好的线性关系,平均加样回收率分别为98.90％,97.62％,97.93％,97.79％.结论：该方法灵敏、可行,重复性好,为布渣叶药材质量控制提供新的参考依据.

Objective：To establish a HPLC method for the content determination of quercetinkaempferol,apigen,isorhamnetin in Microctis Folium.Method：Waters XbridgeTM C1s （4.6 mm ×250 mm,5 μm） was used with a step gradient of methanol-0.4％ phosphoric acid at a flow rate of 1.0 mL ·min-1 The detection wavelength was set at 367 nm,and the column temperature was at 35 ℃.Result：Linear relationships between values of peak area and mass concentration of quercetin kaempferol,apigen,isorhamnetin were kept in the range of 14.58-72.90,63.76-318.8,32.92-164.6,79.68-398.4 ng.The average recovery of quercetin,kaempferol,apigen,isorhamnetin was 98.90％,97.62％,97.93％,97.79％.Conclusion：This method is simple,accurate and can be used for cuality control of Microctis Folium.