不同种类市售海参的总皂苷含量测定

Determination Total Saponin Content in Sea Cucumbers

目的：建立一种海参体壁中总皂苷的含量测定方法,比较我国11种不同种类市售海参和8批格皮氏海参干燥体壁中的总皂苷含量.方法：采用紫外-可见分光光度法,以齐墩果酸为对照品建立标准曲线,5％香草醛-冰醋酸为显色剂,检测波长546 nm,比色法测定总皂苷含量.结果：所建立的方法稳定可靠,不同种海参体壁皂苷含量差异较大,格皮氏海参总皂苷含量最高,市售格皮氏海参皂苷含量在3.16％ ～5.27％之间.结论：方法简便、准确、重复性好,可用于对海参皂苷的含量测定.对我国大部分市售海参种类皂苷含量进行了测定,其中格皮氏海参可以作为一种很好的制备海参总皂苷的原料药材.

Objective：To establish a determination method of the total saponin content in sea cucumber body wall.To compare the total saponin content of dry sea cucumber body wall in 11 kinds of different types of sea cucumber and 8 bitches of Pearsonothuria graeffei.Method：The absorption of the sample solution was measured at 546 nm and the saponin contents were calculated against oleanolic acid by spectrophotometry.vanillin-glacial acetic acid 5％ was used as the chromogenic agent.Result：The method is stable and reliable.Obvious difference was observed in contents between different kinds of sea cucumber body wall.Total saponin content of P.graeffei is high among all species.Saponins contents of the market sales P.graeffei ares between 3.16％-5.27％.Conclusion：The assay method used in this paper is simple,accurate and reproducible,which can be used for saponin content determination of sea cucumber.By the resultwe can have a comprehensive understanding of saponin content of sea cucumbers in Chinese marketing.P.graeffei can serve as a good resource for preparation of sea cucumber total saponins.