酱香型大曲中具产酶功能霉菌的分离筛选

Screening and Isolation of Functional mold Producing Enzyme in Maotai-Flavor Daqu

目的:从酱香型大曲中筛选功能菌。方法:从贵州省某酒厂的酱香大曲中分离到19株霉菌,测定了各菌株的液化酶、糖化酶和蛋白酶活力。结果:筛选到两株同时产液化酶、糖化酶和蛋白酶三种酶活都相对较高的菌株,对两株菌进行鉴定,并构建系统进化树,经鉴定两株菌分别为谢瓦散囊菌FBKL3.0004和阿姆斯特丹散囊菌FBKL3.0013。结论:筛选的两株菌能够应用于白酒酿造。

Aim：Screening functional strains from Maoti-flavor Daqu.Method：We isolated 19 molds in Maotai-falvor Daqu which come from a liquor industry,and detected their α -amylase, glucoamylase and protease. Result：Three enzymes activity screened two molds were higher than others.Then identified them and structured the phylogenetic tree.The FBKL3.0004 was Eurotium chevaleri and the FBKL3.0013 was Eurotium amstelodami.Conclusion：Two molds screened can apply to make liquor.