补阳还五汤对脑缺血后大鼠NF-κB/p50表达的影响

Effect of Buyang Huanwu Decoction on Cerebral NF-κB /p50 Expression of Rats with Focal Cerebral Ischemia Injury

目的:探讨补阳还五汤对脑缺血后大鼠脑组织NF-κB/p50表达的影响。方法:将180只SD大鼠随机分为空白组、假手术组、模型组、补阳还五汤组、PDTC(呲咯烷二硫代氨基甲酸盐)组和米诺环素组,各30只,每组按给药后7天、14天、21天3个时间点再各分为3个亚组,观察各组不同时间点的神经功能评分,免疫组化检测缺血脑组织NF-κB/p50蛋白表达情况。结果:补阳还五汤组、米诺环素组、PDTC组各时间点神经功能缺失症状评分和脑组织NF-κB/p50阳性细胞数与模型组比较,均明显减少(P<0.05或P<0.01);用药7天、14天后,补阳还五汤组NF-κB/p50阳性细胞数与米诺环素组,PDTC组组间差异无统计学意义(P>0.05),用药21天后,补阳还五汤组与PDTC组比较,具有显著性差异(P<0.05)。结论:补阳还五汤可促进脑缺血损伤后神经功能恢复,调控缺血脑组织炎症因子NF-κB/p50的表达可能是其治疗脑缺血的作用机制之一。

Objective：To observe the effect of Buyang Huanwu decoction on NF -κB/p50 expression in brain tissue of rats with focal cerebral ischemia injury .Methods：One hundred and eighty SD rats were randomly di-vided into normal group , Sham -operated group , ischemia group , BYHWD treatment group , PDTC treatment group and MC treatment group .Each group was divided into three subgroups according to the time after admin-istration for 7d,14d,21d respectively.Neurological function score and NF -κB/p50 protein levels were exam-ined by using immunohistochemistry in each group at different time points .Results：Neurological function score and expression of NF-κB/p50 in BYHWD,MC,PDTC treatment group were significantly decreased compared with those in model group at the same time （P〈0.05 or P〈0.01）;after 7,14 days’treatment, the difference of the expression of NF -κB/p50 was not significant （ P〉0.05） .Between the treatment groups ,after 21 days’ treatment BYHWD displayed the trifle superiority in comparison with PDTC （ P〈0 .05 ） .Conclusion：Buyang Huanwu decoction can improve the recovery of cerebral ischemic neurological injury and regulating cerebral in -flammatory cytokine NF-κB/p50 expression of ischemia rats may be one of its therapeutic mechanisms for cerebral ischemia .