产紫杉醇真菌发酵液脱色处理方法

Decolorization of the Fermentation Broth from a Taxol-producing Fungus B19

不同性质的产物发酵后提取纯化工艺技术不同.真菌发酵生产紫杉醇/紫杉烷提取过程中,常常受到色素的干扰,特别是紫杉醇前体10-deacetylbaccatinⅢ(10-DAB)通过HPLC的C_(18)分离柱保留时间与色素峰十分相近,难以分离.为了便于分离纯化产紫杉醇菌株B19发酵液中紫杉醇、10-DAB等成分,研究了B19发酵液的脱色条件.首先比较了大孔树脂NKA-9,AB-8,D3520,颗粒性活性炭和粉末性活性炭等5种材料的脱色效率,发现粉末性活性炭是紫杉醇发酵液的最佳脱色剂.在单因素试验的基础上又进行了正交试验,确定了脱色及10-DAB疑似分子X回收的最优条件:活性炭用量1%(质量体积比),温度60℃,时间30 min,pH 8.0.在此条件下,B19发酵液脱色率为(62.8±0.7)%,X的回收率为(87.2±5.3)%.建立了紫杉烷发酵后的关键除色素步骤,可为类似工作提供借鉴.

In order to separate the putative 10-DAB （10-deacetylbaccatin Ⅲ） from the fermentation broth of B19, a mutant strain of Pestalotiopsis sp. , we investigated the optimal deeolorizaation condition of B19 fermentation broth by activated carbon. We compared the deeolorization efficiency of the Maeroporous resin to that of the activated carbon, and found that powder activated carbon was much more effective for the purpose. The optimal condition determined from single factor test and orthogonal test was as the following： activated carbon 1% （w/v）, temperature 60 ℃, time 30min, pH 8.0. Under this condition, the decolorization rate of B19 fer mentation broth was （62.8±0. 7）%, and the recovery rate of 10-DAB-like molecular was （87.2±5.3）%.