调节性B细胞在慢性乙型病毒性肝炎患者外周血中的表达

Expression of regulatory B cells in peripheral blood of patients with chronic hepatitis B

目的 分析慢性乙型病毒性肝炎（简称慢性乙肝,CHB）患者外周血IL-10＋ CD19＋调节性B细胞（Breg）的表达情况及其临床意义.方法 选取CHB患者61例,其中HBeAg阳性患者26例,对照组30例,抽取EDTA抗凝外周血,分离外周血单个核细胞（PBMCs）,anti-IL-10-PE,anti-CD19-APC抗体进行染色后,流式细胞术分析IL-10＋ CD19＋ Breg的表达情况,同时分析HBeAg阳性和阴性患者Breg表达差异以及Breg与CHB临床指标的相关性;ELISA分析血清IL-10表达水平.结果 CHB和HCs组Breg分别为1.75％（1.20％ ～2.55％）和0.78％（0.55％～1.40％）,CHB组Breg表达高于HCs组,两组差异有统计学意义（Z=-4.54,P＜0.01）;CHB患者中HBeAg阳性患者Breg为2.39％（1.68％～2.86％）,较阴性患者1.28％（1.05％～2.25％）显著升高（Z=-3.354,P=0.001）;CHB和HCs组血清IL-10分别为6.80（5.41～8.42） pg/mL和5.75 （4.40～6.54） pg/mL,两者差异有统计学意义（Z=-2.697,P=0.007）,并且CHB组IL-10与Breg呈正相关关系（r =0.335,P=0.008）.结论 慢性乙肝患者外周血Breg表达显著升高,且可能受到HBeAg的影响.

Objective To investigate the expression of IL-10 ＋ CD19＋ regulatory B cells（Breg） in patients with chronic hepatitis B（CHB）.Methods 61 patients with CHB and 30 healthy controls（HCs）were selected.Peripheral blood mononuclear cells（PBMCs） were isolated from all subjects,and were stained with anti-IL-10-PE,anti-CD19-APC.Flow cytometry was adopted to analyze the expressions of IL-10 ＋ CD19＋ ＋ Breg,and ELISA assay was used to analyze serum IL-10 level.Results Breg in CHB patients and HCs was 1.75％ （1.20％-2.55％）,0.78％ （0.55％-1.40％）,respectively,and the difference between the two groups was statistically significant （Z =-4.54,P ＜ 0.001）.Breg in CHB patients with HBeAg positive was higher than that of negative（Z =-3.354,P =0.001）.Levels of serum IL-10 in patients with CHB and HCs were 6.80 （5.41-8.42） pg/mL and 5.75 （4.40-6.54） pg/mL,and the difference was statistically significant（Z =-2.697,P =0.007）.Serum IL-10 level was positively correlated with Breg in CHB patients （r =0.335,P =0.008）.Conclusion Breg is obviously elevated in patients with CHB and may be affected by HBeAg.