转pprI基因油菜对^(133)Cs或^(88)Sr胁迫的响应

Responses of pprI-transgenic Rape Seedlings to the ^(133)Cs or ^(88)Sr Stress

通过露地盆栽试验,研究了转基因和非转基因油菜幼苗对土壤中不同浓度的133Cs或88Sr单一胁迫的生理响应。结果表明,在不同浓度的133Cs或88Sr处理时,随处理浓度的增加,不管是转基因还是非转基因油菜,其幼苗对133Cs或88Sr的吸收量都增加,其中二者根部的33Cs或88Sr含量均大于地上部分的含量,同时二者根部对88Sr的吸收大于对133Cs的吸收。但是转基因植株根系吸收133Cs或88Sr的含量大于非转基因油菜根系吸收量,转基因油菜地上部分的133Cs或88Sr含量却小于非转基因油菜地上部分的含量,表明转基因油菜根系更易于吸收和固着133Cs或88Sr,并向地上部分进行运输。随核素浓度增加,非转基因和转基因油菜苗叶片中的丙二醛含量逐渐增加,根系活力均表现为不断减弱,幼苗叶片的可溶性蛋白、POD和SOD活性都呈现先上升后下降的趋势;但在相同浓度的133Cs或88Sr处理后,转基因油菜的丙二醛含量总低于非转基因油菜,转基因油菜的可溶性蛋白含量、POD和SOD活性均高于非转基因油菜,同时转基因油菜的根系活力也略高于非转基因油菜。研究表明:转pprI基因油菜比非转基因油菜具有更强的应对133Cs或88Sr胁迫的抗性能力。本研究为进一步研究pprI基因的调控机理及该基因对137Cs成90Sr协迫响应提供理论和实验基础。

The responses of pprI-transgenic and non-transgenic rape seedlings to the different ^133Cs and ^88Sr Single Stress were studied by pot experiments. Result showed that the uptake of ^133Cs or ^88Sr in rape seedlings increased with the increase of ^133Cs or ^88Sr concentration. The ^133Cs or ^88Sr contents of roots were more than that of the up-ground parts in transgenic and non-transgenic rape seedlings,and the88Sr content was higher than the ^133Cs content in the roots. But we found root uptake more ^133Cs or ^88Sr in transgenic rape than non-transgenic rape,and the ^133Cs or ^88Sr content of upground partsin transgenic rape was less than that in non-transgenic rape,which showed that the roots in transgenic rape more readily absorbed and fixed and transported133Cs or ^88Sr to the up-ground part than that in non-transgenic rape.With the increase of ^133Cs or ^88Sr concentration,the MDA content of transgenic and non-transgenic rape leaves were gradually increased,and the soluble protein content,POD and SOD activities were first rose and then declined,but the root activity decreased. Under the same treatment,the MDA content of transgenic rape was lower than that of nontransgenic rape,but the root activity,the content of soluble protein,POD and SOD activity of transgenic rape were higher than those of non-transgenic rape. Therefore,the above results indicated that pprI-transgenic rape has better ability to resist against ^133Cs or ^88Sr than non-transgenic rape. This study will provideces the theoretical and practical basis for further study the regulation mechanism of pprI gene and the gene response to ^133Cs or ^88Sr stress.