电化学发光法检测鲎素

A highly sensitive electrochemiluminescence method for the determination of tachyplesin

基于鲎素（TCP）能强烈抑制钌联吡彤N，N-二丁基乙醇胺（Ru（bpy）3 2＋／DBAE）体系的电化学发光（ECL）信号，建立了检测鲎素的方法。在最优实验条件下，Ru（bpy）3 2＋／DBAE体系的ECL淬灭值△I EcL与TCP质量浓度的对数log P呈良好的线性关系，线性范围为3．0×10 -10～1．5×10 -8g／mL，检出限达50pg／mL（S／N=3）。方法用于鲎试剂样品分析，鲎素的加标回收率为93．0％～107．6％．

A highly sensitive method for the determination of tachyplesin（ TCP）was developed based on its strong eleetrochemiluminescence （ECL） inhibition of Ru （bpy） 3 2 ＋/DBAE system. Under the optimized conditions, the quenched EcL intensity versus the logarithm of the concentration of tachyplesin was linear over a concentration range from 3.0 × 10-10 μg/mL to 1.5 × 10 -8 g/mL. The limit of detection （,LOD） was found to be 5.0 × 10-11 g/mL （S/N = 3 ）. And this method was successfully applied to the estimation of tachyplesin in limulus reagent, with the recoveries in the range from 93.0% to 107.6%.