发酵条件对茂原链霉菌谷氨酰胺转胺酶产量的影响

Effects of fermentation conditions on transglutaminase yield from Streptomyces mobaraense

以茂原链霉菌（Streptomyces mobaraense：DSM40587）为生产菌株,以提高谷氨酰胺转胺酶（TGase）的产量为主要研究目标,通过摇瓶发酵条件的研究,提高了酶的产量,缩短了发酵时间.茂原链霉菌摇瓶生产TGase最终发酵条件为：种龄48 h,接种量8％～12％;培养基组成为：聚蛋白胨30 g/L,可溶性淀粉30 g/L,磷酸氢二钾2 g/L,七水硫酸镁1 g/L,pH=7.0;发酵时间为120 h.

Streptomyces mobaraense （DSM40587） was used for producing transglutaminase, and improving the yield. By studying on the con- dition of shake-flask culture, we improved the yield of transglutaminase and shorted the culture time. The inoculation condition of Strepto- myces mobaraense cultured by shake-flask was 8%-12% inoculation and the stain were cultured for 48 h. The fermentation condition was Er- leumeyer flasks （250 mL） filled with 50 ml of the medium composed of 30 g/L polypeptone, 30 g/L soluble starch, 2 g/L K2HPO4, 1 g/L MgSO4.7H20, pH=7.0, shaken at 200 rpm for 120 h.