多舌飞蓬提取物的急性毒性研究

Acute toxicity study of the extracts from Erigeron multiradiatus

目的研究多舌飞蓬提取物对小鼠的急性毒性。方法根据经典急性毒性试验方法,ig给予小鼠不同浓度的提取物,观察记录小鼠的中毒症状及死亡情况,用改良寇氏法计算LD50及其95%可信限;即刻尸检死亡小鼠,用肉眼观察主要脏器,对有明显病变的器官进行组织病理学检查。结果给药后,小鼠出现呼吸急促、活动减少、俯卧、运动失调、抽搐、呼吸抑制而死亡。LD50为133.71 g·kg-1,95%可信限为121.63～146.98 g·kg-1。肉眼观察发现给药组小鼠的肝脏表面有不同程度的白点,经组织病理学检查发现其主要病变为水肿、脂肪变性及坏死。结论多舌飞蓬提取物具有一定的急性毒性,可造成小鼠肝损伤并导致死亡,临床应用时应控制剂量。

OBJECTIVE To study acute toxicity of the extracts from Erigeron multiradiatus in mice. METHODS According to classical test methods of acute toxicity, the extracts were administered by intragastric administration to mice at varying concentrations. Toxicity symptoms and mortality were observed and recorded, and LD50 and 95% confidence interval were calculated by improved Kar- bet＇ s method. The main organs of dead mice, dissected immediately, were examined by the unaided eye, and then the obviously diseased organs were examined in histopathology. RESULTS The main toxicity symptoms of the mice, which were treated, were polypnea, hypoactivity, pronation, ataxia, convulsions, respiratory inhibition and eventually to death. LD50 and 95 % confidence interval were 133.71 g·kg^-1 and 121.63 - 146.98 g·kg^-1 respectively. The livers of mice,which were treated, were found having different degrees of white spots by the naked eye and their main pathological changes are edema, fatty degeneration and necrosis of hepatic cell via histopathological examination. CONCLUSION The extracts of Erigeron multiradiatus have certain acute toxicity and can cause liver injury and death to mice, so the dose should be controlled in clinical application.