藏紫草的生药学研究及其含量测定

Phamacognosy reaserch and content determination of Zangzicao

目的建立藏紫草的质量标准。方法考证文献、鉴别与测定含量。结果确定了藏紫草为紫草科植物长花滇紫草Onosma hookeri Clarke var.longiflorum Duthie和细花滇紫草Onosma hookeri C.B.Clarke的干燥根,建立了藏紫草TLC鉴别和HPLC测定含量的方法,初步设定了藏紫草所含β,β'-二甲基丙烯酰阿卡宁的含量限度。结论所用方法可显著地提高藏紫草质量的可控性。

OBJECTIVE To establish the quality standards of Zangzicao. METHODS Textual research, TLC and HPLC were used. RESULTS The research demonstrated that the original plants of Zangzicao were the dry roots of Onosma hookeri Clarke vat. longiflorum Duthie and Onosma hookeri C. B. Clarke. The TLC identification method of Zangzicao was established. HPLC was used for the determination of β,β＇ -dimethylacrylalkannin in Zangzicao, and the research established the relative content limitation of β,β＇ - dimethylacrylalkannin in Zangzieao. CONCLUSION The reaserch improved the quality control of Zangzieao.