褪黑素与“肾应冬”理论的关系及机理初探

Relationship between Melatonin and“Correspondence of Kidney with Winter”and its Mechanism

目的通过建立冬季的肾脏生理、病理模型来探讨褪黑素(MT)与"肾应冬"理论之间的联系。方法取清洁级SD大鼠随机分为正常对照组、松果腺摘除伪手术组、松果腺摘除手术组、肾阳虚组。采用酶联免疫(ELISA)法分别检测血清中的Melatonin(褪黑素)、T3(三碘甲状腺原氨酸)、T4(甲状腺激素)的水平。结果松果腺摘除手术组和肾阳虚组大鼠血清中MT、T3、T4的含量明显低于对照组(P<0.01)。与松果腺摘除伪手术组相比较,松果腺摘除手术组和肾阳虚组大鼠血清MT、T3、T4的含量也明显降低(P<0.05)。结论松果腺摘除手术组和肾阳虚组的褪黑素的减少可能对T3、T4的分泌具有抑制作用,肾阳虚组褪黑素分泌的减少使肾应冬的能力下降。

Objective Toexploretherelationshipbetweenmelatonin（MT）andthetheory“corre-spondence of kidney with winter”through setting up the physiological and pathological models kidney in win-ter.Methods Theclean-gradeSDratswererandomizedintoanormalcontrolgroup,apseudo-pinealec-tomy group,a pinealectomy group and a kidney yang deficiency group.ELISA method was adopted to deter-minethelevelsofMT,T3andT4.Results Inthepinealectomygroupandthekidneyyangdeficiencygroup, the levels of MT,T3 and T4 in serum were apparently lower than those in the control group（P〈0.01）.Com-pared with the pseudo-pinealectomy group,the levels of MT,T3 and T4 in serum were also apparently lower thanthoseinthepinealectomygroupandthekidneyyangdeficiencygroup（P〈0.05）.Conclusion The MT decrease in the pinealectomy group and the kidney yang deficiency group probably inhibits the secretion of T3 and T4.The MT decrease in the kidney yang deficiency group reduces the ability of kidney in corre-spondence with the winter.