HPLC-ELSD法测定黄芪中黄芪皂苷Ⅲ、黄芪皂苷Ⅳ的含量

Determination of Astragaloside Ⅲ and Astragaloside IV in Astragalus with HPLC-ELSD Method

目的：建立HPLC-ELSD法测定黄芪中黄芪皂苷Ⅲ和黄芪皂苷Ⅳ的含量。方法采用Dimonsil C18（150 mm ×4．6 mm，5μm）色谱柱，乙腈-水为流动相梯度洗脱，流速为1 mL·min-1，柱温为30℃；ELSD参数：漂移管温度为100℃，空气流速为2．5 L＆#183;min-1，撞击器关闭。结果黄芪皂苷Ⅲ在进样量范围为0．016～1．150μg时，进样量的自然对数与峰面积积分值的自然对数成良好线性关系（r＝0．9992），平均回收率为98．09％，RSD为1．02％（n＝6）。黄芪皂苷Ⅳ在进样量范围为0．093～0．816μg时，进样量的自然对数与峰面积积分值的自然对数成良好线性关系（r＝0．9993），平均回收率为97．39％，RSD为1．13％（n＝6）。结论本方法简便、准确，可用于黄芪的质量控制。

Objective ToestablishHPLC-ELSDmethodforthedeterminationofastragalosideⅢandastragalosideⅣinastragalus.Methods DimonsilC18（150mm×4.6mm,5μm）chromatographiccol-umn was used,acetonitrile-water was as mobile phase for gradient elution,flow rate 1 mL＆#183;min-1 ,column temperature 30 ℃.ELSD parameters：drift tube temperature 100 ℃,air velocity 2.5 L·min-1,impactor closed.Results Insamplesize0.016~1.150μgofastragalosideⅢ,thenaturallogarithmofsamplesize presented good linear relationship with that of peak area value （r =0.999 2 ）,the average recovery was 98.09%,RSD was 1.02%（n=6）.In sample size 0.093 ~0.816 μg of astragaloside Ⅳ,the natural log-arithm of sample size presented good linear relationship with that of peak area value（r=0.999 3）,the aver-agerecoverywas97.39%,RSDwas1.13%（n=6）.Conclusion Thismethodissimpleandaccurateand is applicable for quality control of astragulas.