摘要
目的观察Caspase-3抑制药对甲基苯丙胺(MA)慢性依赖大鼠脑组织损伤的干预与保护作用。方法建立MA慢性依赖大鼠实验动物模型,侧脑室注射Caspase-3抑制药(z-DEVD-fmk),检测各组大鼠脑组织海马区一氧化氮(NO)、超氧化物歧化酶(SOD)和丙二醛(MDA)含量的变化及三者之间的关系。结果 MA慢性依赖组脑组织海马区NO、MDA明显升高,SOD含量减少;MA+z-DEVD-fmk干预组NO、MDA含量升高减少,SOD活性下降减弱,两组比较差异有统计学意义(P<0.01)。结论 Caspase-3抑制药能够降低MA慢性依赖大鼠脑组织海马区NO、MDA含量,并使SOD含量升高,对大鼠脑组织损伤有一定的干预与保护作用。
Objective To study the intervention and protective effect of Caspase-3 inhibitor on damaged brain tissues ofmethamphetamine-dependent rats. Methods The chronic methamphetamine-dependent rat model was established. Caspase-3inhibitor （z-DEVD-fmk） was injected into lateral ventricles to determine the changes of NO, SOD and MDA of the hippocampusin the rats. Results NO and MDA were significantly increased, and SOD decreased in the hippocampus of the chronicmethamphetamine-dependent rats. NO and MDA were slightly increased and SOD slightly decreased in the MA＋z-DEVD-fmk plusmethamphetamine-dependent rats. There was significant difference between the two groups （ P 〈0. 01）. Conclusion Thecaspase-3 inhibitor can reduce the expression of NO and MDA, and increase the expression of SOD in the hippocampus of thechronic methamphetamine-dependent rats. The caspase-3 inhibitor exerts protective effect on rat brain.
出处
《医药导报》
CAS
北大核心
2014年第4期426-428,共3页
Herald of Medicine
基金
辽宁省科技攻关计划项目(2011225020)
