The Prevention and Treatment of Polysaccharide from the Rhizomorph of Armillaria mellea on Diabetic Cataract in Rat

蜜环菌多糖对糖尿病性白内障的防治作用研究(英文)

[Objective] To investigate the prevention and control effect of polysaccharide from the rhizomorph of Armillaria mellea by distilled water elution （AMP-1） on diabetic cataract in rats.[Methods] Streptozotocin （STZ）,combined with freund＇s adjuvant （CFA）,was used to induce diabetic cataract in rat,to observe the onset of lenticular turbidity by slit lamp and compare the turbid degree of eyes lens among different groups.The super oxide dismutase （SOD）,glutathione peroxidase （GSH-px） and malondialdehyde （MDA） in serum and eyes lens were measured by colorimetric method.[Results] The slit lamp examination results showed that AMP-1 obviously delayed the onset of diabetic cataract and reduced turbid degree of eyes lens.Compared with model group,AMP-1 at various doses should significantly reduce the level of MDA ascended in serum of rats.Medium-and high-dose groups could increase the levels of SOD and GSH-Px in serum,and the dose of AMP-1 was closely related to the effect.[Conclusion] AMP-1 played a prominent role in prevention and treatment of diabetic cataract in rats.

[目的]观察蜜环菌多糖(AMP-1)对大鼠糖尿病性白内障的防治作用。[方法]采用链尿佐菌素(STZ)联合完全弗氏佐剂(CFA)诱导大鼠糖尿病性白内障,裂隙灯观察各组晶体出现初次混浊的时间,比较晶体的混浊程度。比色法测定各组血清及晶体中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)含量。[结果]裂隙灯检查结果显示蜜环菌多糖有明显的延缓糖尿病性白内障的发生,减轻晶体混浊程度的作用。与模型组比,AMP-1各剂量组均能明显降低大鼠血清中升高的MDA水平,中、高剂量组可升高血清中SOD、GSH-Px水平,且剂量与效果呈正相关。[结论]AMP-1对大鼠糖尿病性白内障具有显著的防治作用。