双黄连联用左氧氟沙星对兔组织笼感染模型中金黄色葡萄球菌ATCC29213耐药性的影响

Effect of Shuanghuanglian Combined with Levofloxacin on Antibiotic Resistance of Staphylococcus Aureus ATCC29213 in Rabbit Tissue Cage Infection Model

目的探讨双黄连联合左氧氟沙星对兔组织笼感染模型中金黄色葡萄球菌ATCC29213对左氧氟沙星的耐药性的影响。方法建立兔组织笼感染模型,分别用左氧氟沙星单独处理(A组)及双黄连联合左氧氟沙星处理(B组)5 d,探讨兔组织笼感染模型中左氧氟沙星在组织液中稳态浓度及经处理后兔组织笼中细菌恢复生长的情况和细菌耐药性变化。结果两组左氧氟沙星在组织液中浓度差异无统计学意义。B组兔组织笼内细菌恢复生长发生率(20%)明显低于A组(100.0%),而左氧氟沙星对B组兔组织笼内细菌的最低抑菌浓度(MIC)值明显低于A组。结论双黄连与左氧氟沙星联合治疗有助于减少金黄色葡萄球菌对左氧氟沙星的耐药现象,提示中药联合抗菌药物可降低细菌对抗菌药物的耐药性。

Objective To explore effects of shuanghuanglian combined with levofloxacin on antibiotic resistance of Staphylococcus Aureus ATCC29213 to levofloxacin. Methods Tissue cage infection model with Staphylococcus aureus was established in rabbits, and the infected animals were given with levofloxacin alone （group A ） or in combination with shuanghuanglian （group B） for 5 days respectively. Steady-state concentration of levofloxacin in tissue cage, bacteria recovery and bacterial resistance in tissue cage infection model were studied. Results Steady-state concentration of levofloxacin in tissue cage was not significantly different between group A and group B. The recovery rate of bacteria was significantly lower in group B than in group A （20.0% vs. 100.0% ）. The minimum inhibitory concentration （MIC） was lower in group B than in group A. Conclusion Shuanghuanglian combined with levofloxacin is helpful to reduce antibiotic resistance of Staphylococcus aureus to levofloxacin, indicating that some Chinese traditional medicine combined with antibiotics can reduce antibiotic resistance.