理气消瘿片高效液相色谱指纹图谱研究

High Performance Liquid Chromatograph Fingerprint of Liqi Xiaoying Tablets

目的建立理气消瘿片的高效液相色谱(HPLC)指纹图谱,为其质量控制提供依据。方法色谱柱Aichrom Bond-AQ C18(250 mm×4.6 mm,5μm),流动相乙腈-0.1%磷酸溶液(梯度洗脱),柱温30℃,流速1.0 mL·min-1,进样量20μL,检测波长226 nm。结果建立了理气消瘿片的HPLC指纹图谱,共标定了13个共有峰,确认了其中4个共有峰,10批理气消瘿片指纹图谱的相似度均>0.95。结论该方法准确、可靠,可以较全面、完整地反映理气消瘿片的质量。

Objective To establish high performance liquid chromatograph （HPLC） fingerprint of liqi xiaoying tablets,and to provide reference for quality control of the herbal medication. Methods The chromatography conditions consisted of Aichrom Bond-AQ C18（250 min×4.6 mm,5 μm） column with mobile phase of acetonitrile-0.1% phosphoric acid （gradient elution） , column temperature of 30 ℃, flow rate of 1 mL · min^-1, injection volume of 20 μL, and UV detection wavelength of 226 nm. Results HPLC fingerprint was established with 13 common peaks, 4 of which were identified. The similarity of the HPLC fingerprints of liqi xiaoying tablets from 10 batches was greater than 0.95. Conclusion The method is accurate, reliable, and can reflect complete information for the quality of liqi xiaoying tablets.