ACE2基因A9570G多态性与冠状动脉支架内再狭窄的相关性研究

Association between the polymorphism of A9570G in angiotensin converting enzyme 2 gene and restenosis after coronary stent placement

目的探讨ACE2基因A9570G多态性与冠状动脉支架置入术后支架内再狭窄的相关性。方法选择320例成功实施冠状动脉药物洗脱支架置入术的冠心病患者为研究对象,所有患者术后6个月接受冠状动脉造影复查,有支架内再狭窄42例作为再狭窄组,无支架内再狭窄278例作为对照组。应用限制性片段长度多态性技术检测ACE2基因A9570G多态性并比较两组间等位基因频率及基因型分布的差异。结果在女性,再狭窄组G等位基因频率高于对照组,差异有统计学意义(P=0.011),GG基因型分布高于对照组,差异也有统计学意义(P=0.035);在男性,再狭窄组G等位基因频率/G基因型分布高于对照组,差异有统计学意义(P=0.003)。结论 ACE2基因A9570G多态性与冠状动脉药物洗脱支架置入术后支架内再狭窄相关,G等位基因可能是支架内再狭窄的遗传易感因素。

Objective To investigate the relationship between angiotensin converting enzyme 2（ACE2） gene A9570G polymorphism and restenosis after coronary drug-eluting stent.Methods 320 patients were selected underwent intracoronary stenting,all patients were followed up for coronary angiography 6 months after intracoronary stenting,42 patients had restenosis as restenosis group and 278 patients without restenosis as a control group.The polymorphism of ACE2 gene were analyzed by restriction fragment length polymorphism（RFLP） method,and to compare genotype distribution and allele frequency in different groups.Results In the female,G allele frequency in restenosis group was higher than that of control group,the difference was statistically significant（P = 0.011）,the distribution of GG genotype was also higher than that of control group（P =0.035）.In the male,G allele frequency/G genotype distribution in restenosis group was higher than that of control group,the difference was statistically significant（P = 0.003）.Conclusion The polymorphism of A9570G in ACE2 gene is associated with in-stent restenosis,the G allele may be a genetic susceptibility factors of in-stent restenosis.