离子交换-高效液相色谱法测定化妆品中6种水溶性功效成分

Determination of 6 Water-Soluble Compounds in Cosmetics by HPLC-DAD

建立了一种同时测定化妆品中6种水溶性功效成分的分析方法.样品经水直接超声提取后经离子交换色谱柱Rezex ROA-OrganicAcid H＋（8 ％）（300×7.8 mm）分离,10 mmol/L硫酸水溶液作为流动相,流速为0.5 mL/min,二极管阵列检测器进行检测,检测波长分别254 nm和214 nm,外标法定量.在优化实验条件下,6种水溶性功效成分标准曲线线性关系良好,在空白化妆品中的加标回收率为87.3 ％～99.4％,相对标准偏差为2.8 ％～5.7％.

A method was developed for the determination of 6 water-soluble compounds in cosmetics. The sample was extracted by methanol and separated by a RP Cs chromatographic column （4.6×250 mm, 5μm） at a flow rate of 1.0 mL/min. Methanol-water was used as mobile phase. The detection was performed at 325 nm by using high performance liquid chromatography-diode array detection （HPLC-DAD）. The external standard method was employed for quantitative analysis. Under the optimized condition, the average recoveries of spiked samples were between 87.3 % and 99.4 %, with relative standard deviations of 2.8 %-5.7 %.