寻常型银屑病血热证患者外周血Th17细胞活化和增殖相关因子的表达

The Expression of Th17 Cells Activation and Proliferation Related Factors in Peripheral Blood of Patients with Blood-heat Syndrome of Psoriasis Vulgaris

目的探讨Th17细胞活化和增殖相关因子在寻常型银屑病血热证发病机制中的作用以及寻常型银屑病血热证的物质基础与辨证依据。方法收集我院皮肤科门诊寻常型银屑病血热证患者12例为观察组,11例正常人为对照组。通过多色流式细胞术检测两组外周血Thl7细胞活化相关因子HLA-DR、增殖因子Ki-67表达水平。结果观察组外周血Thl7细胞活化因子HLA-DR的表达及增殖因子Ki-67的表达均高于对照组(P<0.05);但活化因子HLA-DR及增殖因子Ki-67与银屑病的病情及严重程度指数(PASI)评分均无相关性(r1=-0.021,r2=0.098,均P>0.05)。结论 Th17细胞活化因子HLA-DR和增殖因子Ki-67在银屑病血热证患者外周血中表达上调,提示银屑病血热证患者外周血Th17细胞处于高活化状态且增殖活跃,二者可能间接参与了银屑病血热证的发病过程,是银屑病血热证新的物质基础与辨证依据。

Objective To explore the role of Th17 cell activation and proliferation related factors in the pathogenesis of psoriasis vulgaris of blood-heat syndrome and material foundation as well as dialectical basis of blood-heat syndrome of psoriasis vulgaris. Methods 12 patients with blood-heat syndrome of psoriasis vulgaris were enrolled from our hospital dermatology clinic, and 11 cases of healthy individuals as normal control. The expression level of Th17 cells activation related factor HLA-DR, proliferation factor Ki-67 in peripheral blood of patients with blood-heat syndrome of psoriasis vulgaris and healthy subjects were examined by multicolor flow cytometry. Results Thelevel of Th17 cells activated factor HLA-DR in peripheral blood of patients was high expression compared with control group （11.686%± 4.799% vs 8.016%± 2.334%, P〈0.05）, the expression of proliferation factor Ki-67 was also increased compared to control group （8.727%± 3.854% vs 5.665%± 2.251%, P〈0.05）. But the activation factor HLA-DR, proliferation factor Ki-67 and psoriasis area and severity index （PASO score did not show correlations （r1=0.021, r2=0.098, P〉0.05）. Conclusion The high expression of Th17 cell activation factor HLA-DR and proliferation factor Ki-67 in peripheral blood of patients with psoriasis of blood-heat syndrome, indicates theperipheral blood of patients with psoriasis of blood-heat syndrome-Th17 cells were at a high state of activation and proliferation. Both HLA-DR and Ki-67 may indirectly involved in the pathogenesis process of psoriasis of blood-heat syndrome. They are probably novel material foundations and dialectical basis of blood-heat syndrome of psoriasis.