两种不同温育方式对酶免试剂检测结果的影响

The influence of two different incubation methods for ELISA detection

目的研究2种不同温育方式对酶免结果的影响。方法将1盒HBsAg试剂平均分成2份,每份均按试剂说明书进行手工加样20孔。1份采用水浴箱加热温育,另1份采用UranusAE200进行加热温育。除温育方式不同外,其余条件均相同,按试剂说明书进行操作。使用340Rt酶标仪读取结果。结果采用水浴箱进行加热,ELISA试验结果为x珋=2.112;采用全自动酶免仪的温育器进行加热,ELISA试验结果为x珋=2.686,经t检验统计分析,P<0.05。结论 2种温育方式对酶免结果的影响具有统计学差异,应建立不同的判别条件,分别进行室内质控分析。

Objective To study the affection of two different incubation methods for ELISA detection. Methods A box of HBV detection reagent were divided into two parts, each part manually add 20 samples according to the reagent manual. One aliquot used water bath for incubation;while the other aliquot used Uranus AE 200 for incubation. The other conditions are all the same for both procedure. The 340RT microplate reader was used to read the results. Results Using water bath incubation method, the ELISA results x = 2.112. Using Uranus AE200 incubation , the ELISA results x = 2.686. T test indicated that using two different incubation methods, ELISA results have statistical significance （ P 〈 0. 01 ） Conclusion The affection of two different incubation methods for ELISA reagent has statistical significance . We should establish different judging conditions to analyze inrernal quality control.