响应面法优化琼胶酶发酵培养基

Agarase Optimization of Fermentation Medium by Response Surface Method

[目的]优化海洋细菌Vibrio agarivorans-1产琼胶酶液体发酵培养基的最佳组分。[方法]首先通过单因素试验筛选出3个重要因子(琼脂、NaCl和酵母膏质量浓度),在单因素的基础上,通过Box-Behnken试验设计和响应面分析法确定了3个因子的最优发酵条件。[结果]最佳营养组分为:琼脂粉质量浓度为3.9 g/L,NaCl质量浓度为45.2 g/L,酵母膏质量浓度为12.9 g/L,此条件下酶活力达到7.589 U/g,比优化前提高了3.35倍。[结论]该研究对提高海洋细菌Vibrio agarivorans-1产琼胶酶具有重要意义。

[Objective] The components of liquid fermentation medium of marine microorganisms Vibrio agarivorans-1 producing agarase were optimized. [ Method ] Three important factors： the concentration of agar, NaC1 and yeast extract in liquid fermentation medium, were screened by single factor experiment. The optimum fermentation conditions were determined by three factors Box-Behnken experimental design and re- sponse surface analysis based on single factor experiment. [ Result] The experimental result indicated that the activity of agarase reached at 7. 602 U/g as the concentration of agar, NaCI and yeast extract was 3.9 g/L, 45.2 g/L and 12.9 g/L, respectively, which was 3.35 times higher than the previous. [ Conclusion] The research has significance for enhancing marine microorganisms Vibrio agarivorans-1 producing agarase.