高效液相色谱法检测(S)-2-氨基丁酰胺盐酸盐的纯度

Purity of (S)-2-Aminobutyramide hydrochloride by high performance liguid chromatograph

用高效液相色谱法定量分析（S）-2-氨基丁酰胺盐酸盐的纯度。用Agilent 1100型高效液相色谱仪，色谱柱Xterra C18（5μm，4.6 mm×250 mm），流动相为20%乙腈-80%，缓冲液：1000 mL水＋5.0 g庚烷磺酸钠；检测波长为210 nm，流量为0.7 mL·min-1，柱温30℃，对（S）-2-氨基丁酰胺盐酸盐纯度进行定量测定。相对标准偏差0.15%，标准回收率99.8%~100.2%，方法重复性好，定量准确度高。

Purity analysis of （S）-2-Aminobutyramide hydrochloride was established by high performance liquid chromatography with Agilent 1100, Xterra C18（5 μm, 4.6 mm×250 mm）column, mobile phase was acetonitrile-water, detection wavelength was 210 nm, flow rate was 0.7 mL·min-1, column temperature was 30℃. The method showed repeatability and relative standard deviation was 0.15%, and the standard recoveries were 99.8%-100.2%. These quantitative results showed high accuracy.