摘要
In this research, the degradation of dibenzothiophene(DBT) was investigated by using Pseudomonas sp. LKY-5 isolated from oil contaminated soil. The response surface methodology(RSM) based on the Box-Behnken design(BBD) was applied for evaluating the interactive effects of four independent variables including substrate concentration, temperature, pH and agitation rate on the DBT removal response. A total of 29 experiments for four factors at three levels were conducted in present study. A second-order regression model was then developed, and the analysis of variance(ANOVA) illustrated that the proposed quadratic model could be utilized to navigate the design space. The value of determination coefficient(R2=0.953 4) indicated a satisfactory agreement between the quadratic model and the experimental data. It was found that DBT removal was more significantly affected(P<0.000 1) by substrate concentration compared with other three parameters. An 100% degradation of DBT could be obtained by Pseudomonas sp. LKY-5 at a substrate concentration of 100 mg/L.
In this research, the degradation of dibenzothiophene （DBT） was investigated by using Pseudomonas sp. LKY-5isolated from oil contaminated soil. The response surface methodology （RSM） based on the Box-Behnken design （BBD）was applied for evaluating the interactive effects of four independent variables including substrate concentration, temperature,pH and agitation rate on the DBT removal response. A total of 29 experiments for four factors at three levels wereconducted in present study. A second-order regression model was then developed, and the analysis of variance （ANOVA）illustrated that the proposed quadratic model could be utilized to navigate the design space. The value of determination coefficient（R2=0.953 4） indicated a satisfactory agreement between the quadratic model and the experimental data. It was found thatDBT removal was more significantly affected （P〈0.000 1） by substrate concentration compared with other three parameters.An 100% degradation of DBT could be obtained by Pseudomonas sp. LKY-5 at a substrate concentration of 100 mg/L.
基金
support provided by the Fundamental Research Funds for the Central Universities (No. 12CX06043A) of China
the Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology,SOA(No. 201407)
