枯草芽孢杆菌Dg5041液体发酵生产γ-聚谷氨酸

Production of Poly-γ-glutamic Acid by Bacillus subtilis Dg5041 through Liquid Fermentation

以枯草芽孢杆菌Dg5041为生产菌,对其摇瓶发酵生产γ–聚谷氨酸(γ-PGA)的工艺进行研究,通过单因素实验和正交实验获得了该菌的优化培养条件。优化的培养基组成为:葡萄糖35 g/L,酵母膏10 g/L,谷氨酸钠40 g/L,MgSO41.0 g/L,K2HPO4 2.0 g/L,MnSO40.5 g/L,pH 7.0,250 mL锥形瓶装液量50 mL。菌种在37℃,120 r/min培养24 h加入5%NaCl后继续培养24 h,γ-PGA产量达到18.54 g/L。研究结果表明,枯草芽孢杆菌Dg5041是一株很有潜力的γ-PGA高产菌。

Poly-γ-glutamic acid （y-PGA） was produced by using Bacillus subtilis Dg5041 through liquid fermentation processes in shake flask. Through the single factor experiments and orthogonal experiments, the optimized culture was composed of 35 g/L glucose, 10 g/L yeast extract, 40 g/L sodium glutamate, 1.0 g/L MgSO4, 2.0 g/L K2HPO4, 0.5 g/L MnSO4, with initial pH at 7.0, culture volume 50 mL in 250mL conical flask.The production of y-PGA reached 18.54 g/L with B. subtilis Dg5041 being cultivated at 37℃, 120 r/min for 24 h, and a continual cultivation for 24 h after adding 5 % NaC1. The results show that B. subtilis Dg5041 is a high-yielding bacterium for production of γ-PGA.