摘要
目的:探讨不同类型音乐对老年大鼠脑组织海马区Caspase-3和Livin表达水平的影响。方法:取健康老年雄性Wistar大鼠40只,随机分为:空白对照组、莫扎特音乐组、梁祝音乐组和摇滚音乐组,每组各10只;每天上午水迷宫训练同时音乐干预2 h,下午单纯音乐干预2 h,连续7天,第8天处死大鼠取血液及脑组织,采用ELISA检测大鼠血清及脑组织中Caspase-3和Livin蛋白含量的变化水平;采用免疫组织化学法检测大鼠脑组织海马区中Caspase-3和Livin蛋白表达水平;采用实时-PCR检测大鼠脑组织中Caspase-3和Livin的基因变化水平。结果:与对照组比较,莫扎特音乐组、梁祝音乐组大鼠血清及脑组织中Caspase-3蛋白含量、蛋白表达和基因水平均降低有统计学意义(P<0.01),Livin蛋白含量、蛋白表达和基因水平均升高有统计学意义(P<0.01),莫扎特音乐组略好于梁祝音乐组差异无统计学意义(P>0.05);而摇滚音乐组大鼠血清及脑组织中Caspase-3和Livin蛋白含量、蛋白表达和基因水平均无差异无统计学意义(P>0.05)。结论:莫扎特音乐组和梁祝音乐组能通过降低血清及脑组织中Caspase-3水平、提高Livin水平,抑制机体脑神经细胞的凋亡,从而起到神经保护作用;而摇滚音乐组在本研究中效果不明显。
Objective:To explore the effects of different types of music on Caspase-3 and Livin expression levels of the hippocampus of brain tissue of aged rats.Methods: Healthly elderly male Wistar rats 40 were randomly divided into :control group , Mozart music group ,Liangzhu music group and rock music group ,n=10;water maze training every morning while music intervention 2 h,afternoon pure music intervention 2 h,continuous 7 d,the eighth days ,the rats were sacrificed and taking blood and brain tissue ,by ELISA in serum and brain tissue Caspase-3 and Livin protein contents change;using immunohistochemistry assay in rat brain tissue Caspase-3 and Livin protein levels;real-time PCR detection of rat brain tissue changes in gene level of Caspase-3 and Livin.Results:Compared with the control group ,Mozart music group ,Liangzhu music rats the protein content ,protein level and gene level of Caspase-3 of serum and brain tissue were lower statistically significant ( P〈0.01 ) , Livin was increased there was statistically significant ( P〈0.01 ) ,Mozart music group and Liangzhu music group were no significant difference ( P〉0.05 );while rock music group the protein content,protein level and gene level of Caspase-3 and Livin of brain tissue were not significantly difference (P〉0.05).Conclusion:Mozart music group and Liangzhu music group by reducing serum and brain tissue Caspase -3 and increased Livin , to inhibit the apoptosis of the body′s nerve cells,which play a neuroprotective effect;the rock music group in the present study ineffective.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2014年第7期898-900,908,共4页
Chinese Journal of Immunology
基金
吉林省教育厅基金资助(2012460)