外周血单个核细胞介导的HBV宫内垂直传播

Intrauterine vertical transmission of HBV via pathway of peripheral blood mononuclear cells

目的:探讨HBV感染外周血单个核细胞在介导乙肝病毒母婴传播中的作用。方法:以Ficoll-Hypaque常规分离、纯化孕妇外周血单个核细胞(PBMCs)和新生儿脐血单个核细胞(CBMCs),PCR法检测孕妇外周血和新生儿脐血HBVDNA。结果:HBeAg(+)孕妇血清和PBMCs内HBV-DNA检出率分别为100.00%(25/25)和72.00%(18/25),其新生儿脐血血清和CBMCs内HBV-DNA检出率分别为60.00%(15/25)和44.00%(11/25),与HBeAg(-)组相比差异有显著性(P<0.05)。PBMCs内HBV高复制组其新生儿脐血及CBMCs内HBV-DNA阳检率均较高,与其他组相比,差异有显著性(P<0.05)。结论:HBV不仅可通过血清介导HBV宫内垂直传播,还可侵染外周血单个核细胞,并通过外周血单个核细胞→脐血单个核细胞途径介导HBV宫内垂直传播。

Objective：To study the HBV infection in peripheral blood mononuclear cells in mediating the role of mother -to-child transmission of hepatitis B virus.Methods： The peripheral blood mononuclear cells （ PBMCs ） in maternal and cord blood mononuclear cells （ CBMCs ） in newborns were conventionally isolated by Ficoll-Hypaque medium.The loads of HBV-DNA in peripheral blood of maternal and cord blood of newborns were both detected by PCR .Results：The clinical data showed that the positive detection rates of HBV-DNA in serum and PBMCs of pregnant women with HBeAg （＋） were 100.00%（ 25/25 ） and 72.00%（ 18/25）,and the positive detection rates of HBV-DNA in the neonatal umbilical cord blood serum and CBMCs were 60.00%（15/25） and 44.00%（11/25）,respectively.There were significantly difference between HBeAg （＋） and HBeAg（-） in the pregnant women （P〈0.05 ）.The positive detection rates of HBV-DNA in neonatal umbilical cord blood serum and CBMCs were higher in the group with high HBV loads （more than 106copies/ml） in PBMCs than those of low HBV loading group （102-103copies/ml）.The significantly difference was explored between the two groups.Conclusion： Mononuclear cells can not only be infected by HBV , but also play a critical role in the intrauterine vertical transmission of HBV via the pathway transmitted from PBMCs in pregnant women to CBMCs in newborns.