沙棘果渣中异鼠李素超声辅助提取工艺研究

Extraction of isorhamnetin from marc of Hippophae rhamnoides L. using ultrasound-assisted method

采用超声波法提取沙棘果渣中异鼠李素。首先采用单因素对超声频率、提取时间、次数、提取温度、提取料液比及提取溶剂进行筛选,再对筛选提取条件进行正交验证试验。确定了该方法提取沙棘果渣中异鼠李素的参数,即50℃,超声频率40 kHz的条件下用V乙酸乙酯:V95%乙醇为4:6的提取液超声2次,每次1 h,料液比1:25,所得的异鼠李素含量可达5.09 mg·g-1。

The ultrasound-assisted method was used to extract isorhamnetin from marc of the Hippophae rhamnoides fruits. A single factor experiment was conducted to select ideal ultrasonic frequency, extraction time and frequency, extraction temperature, solid-liquid ratio, and extraction solvent. These extraction conditions were then verified with an orthogonal experiment. The best conditions of the ultrasonic method for isorhamnetin extraction from Hippophae rhamnoides marc were： the ultrasonic frequency of 40 kHz, the extraction temperature of 50 ℃, V ethyl acetate： V95% ethyl alcohol of 4：6 as the extraction solvent, two extractions with one hour each, and the solid-liquid of 1：25. Under these conditions, the yield of isorhamnetin extraction can reach 5.09 mg·g^-1.