摘要
采用超声波法提取沙棘果渣中异鼠李素。首先采用单因素对超声频率、提取时间、次数、提取温度、提取料液比及提取溶剂进行筛选,再对筛选提取条件进行正交验证试验。确定了该方法提取沙棘果渣中异鼠李素的参数,即50℃,超声频率40 kHz的条件下用V乙酸乙酯:V95%乙醇为4:6的提取液超声2次,每次1 h,料液比1:25,所得的异鼠李素含量可达5.09 mg·g-1。
The ultrasound-assisted method was used to extract isorhamnetin from marc of the Hippophae rhamnoides fruits. A single factor experiment was conducted to select ideal ultrasonic frequency, extraction time and frequency, extraction temperature, solid-liquid ratio, and extraction solvent. These extraction conditions were then verified with an orthogonal experiment. The best conditions of the ultrasonic method for isorhamnetin extraction from Hippophae rhamnoides marc were: the ultrasonic frequency of 40 kHz, the extraction temperature of 50 ℃, V ethyl acetate: V95% ethyl alcohol of 4:6 as the extraction solvent, two extractions with one hour each, and the solid-liquid of 1:25. Under these conditions, the yield of isorhamnetin extraction can reach 5.09 mg·g^-1.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2014年第4期575-578,共4页
Journal of Anhui Agricultural University
基金
贵州省科技计划项目[黔科合SY字[2013]3014]资助
关键词
沙棘果渣
异鼠李素
提取
marc of Hippophae rhamnoides L.
isorhamnetin
extraction