两种愈伤组织生理生化特性和形态结构比较

Characteristics of Physiology and Biochemistry and Morphological Structure of Two Types of Callus

为了解愈伤组织形成单细胞克隆体系的关键因素,以青睐苜蓿为试材,比较了两种形态的愈伤组织的生理生化指标和形态结构变化。结果表明,两种类型的愈伤组织细胞膜透性和丙二醛含量无明显差异;细胞内可溶性糖含量和过氧化物酶POD活性差异较大,其中可溶性糖含量为:松散型>巨大型,松散型是巨大型的2.09倍;POD酶活性:巨大型>松散型,巨大型是松散型的1.35倍;巨大型愈伤组织细胞较大、组织排列疏松、细胞高度液泡化、细胞质较稀薄,而松散型愈伤组织细胞较小、组织排列松散、细胞液泡较小、细胞质较浓。巨大型与松软型愈伤组织细胞的膜代谢程度相同,但松散型愈伤组织细胞中糖积累多,细胞代谢旺盛;而巨大型愈伤组织糖积累少,细胞衰老。

In order to learn the key factors of callus development on single cell clone, characteristics of physiology and biochemistry and morphological structure of two types of alfalfa callus was studied. The results showed that the cell membrane permeability and malondialdehyde content was same for the two types of callus; but their soluble sugar content and peroxidase activity in cells was very different, for the soluble sugar content： soft callus 〉 huge callus, and soft callus was 2.09 times of huge callus; for pemxidase activity： huge callus 〉 soft callus, and huge callus was 1.35 times of soft callus. Huge callus cells were larger, loosely arranged, cell vacuolization and cytoplasm was thiner than soft callus. However, soft callus cells were smaller, loosely arranged, cell vacuoles was smaller and cytoplasm was thicker than hug callus. Huge cells had the same membrane metabolic with soft ceils, but soft cells had higher sugar accumulation than huge cells, and soft cells metabolism were vigorous, while huge cells were more aging.