聚乳酸-乙醇酸/纳米氧化锌复合电纺纤维装载亲疏水药物的控释及体外细胞毒性

Controlled Release of Multiple Hydrophilic and Hydrophobic Drugs and in vitro Cytotoxicity of Electrospun Poly( lactic-co-glycolic acid) /ZnO Nanofibers Encapsulated with Dual Drugs

利用静电纺丝技术制备了负载亲水性药物阿霉素(DOX)以及疏水性药物喜树碱(CPT)的复合纳米纤维.先用巯基封端的普朗尼克(F127)修饰纳米氧化锌(FZnO),再将FZnO负载盐酸阿霉素(DOX@FZnO),最后将DOX@FZnO与CPT一起纺入聚乳酸-乙醇酸(PLGA)纤维中.体外药物释放结果表明,复合纳米纤维能够减小亲水性药物的突释,减缓药物释放速率,延长药物释放时间.体外细胞活性结果表明,双载药复合纤维比单载药复合纤维具有更强的细胞毒性,能够有效抑制癌细胞生长.

A novel poly（ lactic-co-glycolic acid） （ PLGA）/ZnO electrospun composite fibers encapsulated with both hydrophilic drug（doxorubicin hydrochloride, DOX） and hydrophobic drug（camptothecin, CPT） were fabricated via electrospinning method. Primarily, the ZnO was decorat with F127 and then used to encapsulate DOX. Finally, the DOX-loaded ZnO（ DOX@ZnO） and CPT were mixed with PLGA solution to fabricate elec-trospun hybrid nanofibers. The in vitro release results demonstrated the composite fibers decreased the burst release and increased the time of release, which showed a long-term and sustained release. The cell cytotoxici-ty test demonstated that the composite nanofiber with two drugs showed stronger antitumor efficacy against HepG-2 cells than the nanofiber with single drug. Thus, the composite nanofibers with two anticancer drugs could be a versatile drug delivery system as local implantable scaffolds for potential postsurgical cancer treat-ment.