广西肝癌家族CYP3A5基因多态性的研究

A Study on Gene Polymorphism of Cyp3a5 in Guangxi Liver Cancer Family

目的探讨CYP3A5基因多态性与广西肝癌遗传易感性的关系。方法在广西肝癌高发区选取18个肝癌高发家族成员178例(高发组)及与之相对应的18个无肿瘤家族成员178例(对照组)为研究对象,用流行病学问卷调查方法收集两组相关资料,同时现场采集空腹外周静脉血10 ml,用多聚酶链-限制性片段长度多态性技术(PCR-RFLP)检测CYP3A5基因型。结果高发组GG基因型比例低于对照组(P<0.01),GA+AA基因型比例高于对照组(P<0.05)。高发组A等位基因比例高于对照组(P<0.05)。结论 CYP3A5基因多态性与广西肝癌的遗传易感性密切相关。

Objective Objective To investigate the relationship between genetic polymorphism of CYP3A5 and genetic susceptibility of hepatocellular carcinoma（ HCC） in Guangxi. Methods One hundred and seventy-eight cases were collected from 18 HCC area of the high HCC incidence in Guangxi as high-risk group,and another 178 cases from 18 families with non-tumor history as control group. The relevant data of two groups were obtained through the epidemiological questionnaires. Concurrently,10 ml fasting peripheral blood was collected. The genotype of CYP3A5 was analyzed by polymerase chain reaction-restriction fragment length polymorphism（ PCR-RFLP）. Results The genotype ratio of GG was lower but of GA ＋ AA was higher in the high-risk group in contrast with those in the control group（ P〈0. 05）. There was statistical difference in the A allele ratio between high-risk group and control group（ P〈0. 05）. Conclusion The gene polymorphism of CYP3A5 is closely associated with genetic susceptibility of HCC in Guangxi.