系统性红斑狼疮患儿外周血滤泡辅助性T细胞相关细胞因子及受体表达水平研究

Study of follicular helper T lymphocytes related cytokines and receptors in children with systemic lupus erythematosus

目的研究系统性红斑狼疮(SLE)患儿外周血滤泡辅助性T细胞相关细胞因子及受体表达水平。方法用ELISA检测22例SLE患儿和20例年龄、性别匹配的健康儿童(对照组)血清中的滤泡辅助性T细胞相关细胞因子(CXCL13、IL-21)的水平,采用流式细胞仪检测滤泡辅助性T细胞相关受体(CXCR5、IL-21R)在两组儿童外周血CD19+B细胞中的表达。结果SLE患儿血清中CXCL13、IL-21的水平显著高于对照组(P<0.01)。CXCR5在SLE患儿外周血B细胞上的表达显著低于对照组(P<0.05),IL-21R在两组对象中差异无统计学意义(P>0.05)。结论 SLE患儿血清中CXCL13、IL-21的水平升高提示滤泡辅助性T细胞在SLE患儿体内活性增高,对自身反应性B细胞可以产生过强的辅助信号,滤泡辅助性T细胞在SLE患儿的发病机制中起着重要的作用。

Objective To study the change of the plasma concentrations of follicular helper T lymphocytes （TFH cells） related cytokines and receptors in children with systemic lupus erythematosus （SLE） .Methods The plasma concentrations of TFH-cells-related cytokine IL-21 and CXCL13 ,and cell surface expression of TFH-related receptor CXCR5 and IL-21R on CD19＋B cells in 22 SLE children and 20 sex-and age matched control subjects were measured by enzyme-linked immunosorbent assay and flow cytome-try ,respectively .Results Plasma CXCL13 and IL-21 concentrations were significantly higher in SLE children than controls （all P〈0 .01） .Cell surface expression of CXCR5 on B cells was significantly lower in SLE children than controls （P〈0 .05） .However ,the cell surface expression of IL-21R had no significant changes between SLE children and controls（P〉0 .05） .Conclusion The elevat-ed production of CXCL13 and IL-21 may be associated with the function of TFH for the immunopathogenesis in SLE children .