氟虫腈高效液相色谱分析方法优化

Optimization on Method of HPLC for Fipronil

【目的】对农药中添加的氟虫腈进行高效液相色谱分析条件的优化。【方法】用二级管阵列检测器，C18柱为固定相，以乙腈-甲醇-0．05％磷酸为流动相（体1：L45：20：35）。【结果】该方法分离效果较好，检出限0．5mg／L，平均回收率为98．6％－102．8％。【结论】该方法检出限低，回收率高，可满足各种类型农药中氟虫腈的检测。

[Aims] Method of high performance liquid chromatography for fipronil in pesticide products was studied. [Mothods] The method was conducted by using acetonitrile-methanol-0.05% ortho-phosphoric acid as mobile phase （45： 20：35, by vol）, octadecylsilyl silica column as stationary phase and diode array detector. [Results] The identification was distinct in the method, and the detection limit was 0.5 mg/L. The average recovery rate was 98.6 to 102.8%. [Conclusions] The method had low detection limit and high recovery rate, can meet the demands of the detection of fipronil in various types of pesticide products.