HPLC法同时测定垂盆草片中槲皮素等3种成分

Simultaneous determination of three chemical components in Chuipencao Tablets by HPLC

目的建立同时测定垂盆草片中槲皮素、山奈素、异鼠李素3种成分的HPLC方法。方法采用Imertsit ODS-SP色谱柱（150×4．6mm，5μm），柱温35℃，流动相为甲醇旬．4％磷酸溶液（45：55），流速1．0mL·min^-1，检测波长360nm．结果3种成分均达到基线分离，槲皮素、山奈素、异鼠李素3种成分分别在0．94μg·mL^-1～37．6μg·mL^-1，0．808μg·mL^-1-16．16μg·mL^-1，1．116μg·mL^-1～22．32μg·mL^-1。的浓度范围内与峰面积线性关系良好。平均回收率分别为101．95％、99．01％、101．00％。结论该方法准确可靠，可用于垂盆草片的质量控制。

OBJECTIVE To establish a HPLC method for simultaneous determination of three chemical components （quercetin,isorhamnetin,kaempferol） in Chuipencao Tablets. METHODS The HPLC was performed on an Intertsil ODS-SP column （ 150 × 4.6mm, 5 μm） at 35 ℃ with methol-0. 4% phosphoric acid solution （45：55） as the mobile phase, and detected at 360nm with flow rate of 1.0mL · min-1. RESULTS Three compounds were separated perfectly, linearities of quercetin, isorhamnetin, kaempferol were in ranges of 0. 94μg · mL^-1 - 37.6μg · mL^-1, 0. 808μg · mL^-116. 16μg · mL^-1 ,1. 1.16μg · mL^-1 -22. 32μg · mL^-1 ,respectively. The average recoveries were 101.95% ,99.01% ,101.00%, respectively. CONCLUSION The method is accutae and reliable for the quality control of Cuipencao Tablets.