摘要
目的建立HPLC法测定疏风解毒胶囊大黄素的含量。方法采用Kromasil C18(250mm×4.6mm。5μm)色谱柱,柱温:25℃,流动相:甲醇旬.1%磷酸溶液(75:25),流速:1.0mL·min^-1,检测波长:254nm。结果大黄素进样量在0.04576-0.6864μg范围内呈良好线性关系(r=0.9998),平均加样回收率为99.59%(n=6)。结论本法操作简便、准确、灵敏,可有效控制疏风解毒胶囊中大黄素的含量。
OBJECTIVE To establish a HPLC method for determination of Emodin in Shufeng Jiedu Capsules. METHODS Kromasil C18 (250mm - 4. 6mm, 5μm) Column was used, the column temperature was 25℃, The mobile phase consisted of menthanol-phosphoric solution ( 0. 1% ) ( 75 : 25 ) and the flow rate was 1.0mL · min^-1,The detection wavelength was 254nm. RESULTS emodin has a good linear relation in the rang of 0. 04576 -0. 6864μg(r =0. 9998) ,the average recovery(n =6) was 99.59%. CONCLUSION This method is simple,accurate, sensitive. It can effectively control the content of Emodin in Shufeng jiedu Capsules.
出处
《海峡药学》
2014年第7期57-59,共3页
Strait Pharmaceutical Journal