粒细胞集落刺激因子对脑出血大鼠血管新生的影响及其作用机制研究

Effect of Granulocyte Colony-stimulating Factor on Angiogenesis in Rats with Intracerebral Hemorrhage and Its Mechanism

目的探讨粒细胞集落刺激因子(G-CSF)对脑出血(ICH)大鼠血管新生的影响及其作用机制。方法选取健康SD大鼠63只,随机分为假手术组、ICH组、治疗组,每组21只。制备ICH模型,治疗组大鼠造模1 h后腹腔注射重组人粒细胞集落刺激因子注射液60μg/kg,假手术组、ICH组大鼠经腹腔注射等体积的0.9%氯化钠溶液。比较3组大鼠于造模后6 h、24 h、48 h、72 h、7 d、14 d、21 d神经功能障碍评分、CD34阳性细胞数、血管内皮生长因子(VEGF)阳性细胞数。结果 ICH组大鼠造模后6 h、24 h、48 h、72 h、7 d、14 d、21 d神经功能障碍评分均低于假手术组,治疗组大鼠造模后24 h、48 h、72 h、7 d、14 d、21 d神经功能障碍评分均高于ICH组(P<0.05)。ICH组大鼠造模后6 h、24 h、48 h、72 h、7 d、14 d、21 d CD34阳性细胞数均多于假手术组,治疗组大鼠造模后6 h、24 h、48 h、72 h、7 d、14 d、21 d CD34阳性细胞数均多于ICH组(P<0.05)。ICH组大鼠造模后6 h、24 h、48 h、72h、7 d、14 d、21 d VEGF阳性细胞数均多于假手术组,治疗组大鼠造模后6 h、24 h、48 h、72 h、7 d、14 d、21 d VEGF阳性细胞数均多于ICH组(P<0.05)。结论 G-CSF可促进ICH大鼠损伤区域血管新生及神经功能恢复,其作用机制可能为上调CD34及VEGF的表达。

Objective To investigate the effect of granulocyte colony-stimulating factor（ G-CSF） on angiogenesis in rats with intracerebral hemorrhage（ ICH） and its mechanism. Methods A total of 63 healthy Spragne-Dawley rats were se-lected and randomly divided into groups A（ given sham-operation）,B（ made ICH models）,C（ made ICH models）,each of 21 cases. Then group C given rhG-CSF 60 μg/kg by intraperitoneal injection after 1 h of models made,while groups A and B given equal volume of 0. 9% sodium chloride solution. Neural function defection score,positive CD34 cell counts and positive VEGF cell counts were compared at 6 h,24 h,48 h,72 h,7 d,14 d,21 d after models made in the three groups. Results Neural function defection score of group B was significantly higher than that of group A,at 6 h,24 h,48 h,72 h,7 d,14 d,21 d after models made,respectively;and neural function defection score of group C was significantly higher than that of group B,at 24 h,48 h,72 h,7 d,14 d,21 d after models made,respectively（P﹤0. 05）. Positive CD34 cell counts and positive VEGF cell counts of group B were significantly higher than those of group A,at 6 h,24 h,48 h,72 h,7 d,14 d, 21 d after models made;and positive CD34 cell counts and positive VEGF cell counts of group C were significantly higher than those of group B,at 6 h,24 h,48 h,72 h,7 d,14 d,21 d after models made. Conclusion G-CSF could promote the angiogenesis and improve the recovery of neural function,and its possible mechanism is upregulating the expression of CD34 and VEGF.