顶空进样法和水蒸汽蒸馏法提取佛手挥发性成分分析

Analysis of Volatile Components in Bergamot Collected by Headspace Sampling and Steam Distillation

目的:分析不同的采集方法获得的佛手挥发性成分的差异。方法:分别采用水蒸汽蒸馏法和顶空进样法采集佛手挥发性成分,并对样品进行GC-MS分析。结果:两种采集方法获得的成分有一定的差异性,水蒸汽蒸馏法共鉴定出19种成分,顶空进样法共鉴定出17种成分,共有成分有13种,其中这两种采集方法获得的两种含量最高的组分相对含量基本一致。结论:顶空进样法采集到的结构种类更多,联合使用这两种方法可获得佛手挥发性成分更全面的结构信息。

Objective： To compare the difference of volatile components in bergamot by different sample collection methods. Methods： Volatile components in bergamot were collected by headspace sampling and steam distillation and analyzed by GC-MS. Results： There were some differences between the above methods. There were 19 components identified from extract by steam distillation; whereas 17 components were identified from extract by headspace sampling. There were 13 common components a- mong the identified compounds in the samples collected with different method and the relative contents of the two most impor- tant components were basically identical. Conclusion： More variety of components can be identified in the samples collected with headspace sampling. More volatile components could be identified if the two collection methods were combined.