摘要
目的研究褪黑素(melatonin,MT)对阿尔茨海默病(Alzheimer’s disease,AD)的胆碱乙酰基转移酶(Chat)表达特征的影响。方法大鼠随机分为AD组、假AD组、AD-MT大剂量干预组和AD-MT小剂量干预组,应用β-淀粉样蛋白(Aβ)注入大鼠海马CA1区,建立大鼠AD模型,AD-MT干预组以MT灌胃,1次/d,直至试验结束。应用电迷宫检测大鼠学习记忆情况,用免疫组织化学SABC法检测海马Chat表达特征。结果 MT可以明显改善AD大鼠的学习记忆能力,同时抑制因Aβ在脑内的沉积所导致的海马Chat表达减少。结论 MT对AD有显著的脑保护作用。
Objective To study the expression of Chat in the rats with Alzheimer ＇s disease（AD）in the use of melatonin （MT）.Methods Rats were divided into 4 groups randomly ,AD group ,AD-MT big dosage treatment group ,AD-MT little dosage treatment group and false AD group. To establish AD rat models ,Aβwas injected into the rats’CA1 of sea horse re-gion ,at the same time the AD-MT treatment group was fed with MT everyday untill the experiment ended. Then electricial maze was used to detect their abilities of learning and memory and the immunohistochemical technique was used to detect the expression of Chat.Results MT may improve the abilities of learning and memory of AD rats obviously ,at the same time it may inhibit the decrease of the expression of Chat which was induced by the deposition of Aβin the brain.Conclusion MT has obvious protection for brain with AD.
出处
《中国实用神经疾病杂志》
2014年第14期3-4,共2页
Chinese Journal of Practical Nervous Diseases
