染色体C带荧光染色鉴定实验鼠卵细胞非整倍体

Identification of Aneuploidy with Fluorescence C-banding Staining in Mouse Oocytes

目的:探讨对实验鼠卵母细胞染色体C染色的简易方法。方法:分别采用染色体C带吉姆萨染色方法和改良的染色体C带吉姆萨染色方法后,对实验鼠卵母细胞直接采用4,6-二脒基-2-苯基吲哚(DAPI)进行染色体C带荧光染色法,通过荧光显微镜观察染色体的形态与数量。结果:染色体C带吉姆萨染色方法的染色弱致使染色体分辨率低,改良后的染色体C带吉姆萨染色法易导致染色体溶解,而染色体C带荧光染色法不仅增加了染色体着丝粒和淡染的染色体臂的对比度,而且缩短了染色时间。结论:染色体C带荧光染色法是鉴别实验鼠卵细胞正常染色体与非整倍性染色体的简易方法。

Objective：To investigate the oocyte aneuploid in mice with the method of chromosome C- banding staining. Methods：The standard Giemsa C-banding staining and the modified Giemsa C-banding staining were used to show mouse chromosomes, respectively. The DAPI （4＇, 6-diamidino-2-phenylindole） staining, so-called fluorescence C-banding staining, was used to show oocytes. Subsequently, the morphology and number of chromosomes were analyzed with fluorescence microscope. Results：Chromosomes were weakly stained by the standard Giemsa C-handing staining, while chromosomes were easily dissolved by the modified Giemsa C-banding staining. The fluorescence C-banding staining showed superiorities, such as the increased contrast degree between the brilliant white centromeres and the strong blue arms of chromosomes, and the shorten dyeing time. Conclusious：The study suggested that the fluorescence C-banding staining was a simple and rapid method to identify aneuploidy of mouse oocytes.