CMIA法在梅毒血清学检测中的应用评价

Evaluation of the Application of CMIA Method in Serological Detection of Syphilis

目的探讨化学发光微粒子免疫分析(CMIA)法在TP感染血清学检测中的临床应用。方法收集该院2013年7月—2013年11月门诊及住院部经CMIA法检测疑似梅毒阳性(S/CO≥1.0)标本270例进行临床研究,并对阳性标本采用TPPA和TP-ELISA法进行检测。结果 270例CMIA法阳性标本中,TPPA阳性检出率81.85%(221/270),TP-ELISA阳性检出率83.33%(225/270)。TPPA、TP-ELISA与CMIA对比有差异有统计学意义,高、中浓度对比分析差异无统计学意义,低浓度对比分析差异有统计学意义。结论 CMIA法较TPPA和TP-ELISA法具有更高的特异性及敏感性,用于梅毒螺旋体特异性抗体筛查有助于提高准确性与阳性检出率。

Objective To investigate the clinical application of chemiluminescent microparticle immunoassay （CMIA） in serological detection of TP infection. Methods 270 cases of specimens suspected with syphilis positive （S/CO≥1.0） detected by CMIA method in the Outpatient Department and Inpatient Department of our hospital from July, 2013 to November, 2013 were collected for the clinical study. And the positive specimens were detected by TPPA and TP-ELISA method. Results Of the 270 cases of positive specimens detected by CMIA method, the positive detection rate of TPPA was 81.85%（221/270）, and that of TP-ELISA was 83.33%（225/270）. The difference between TPPA, TP-ELISA and CMIA was statistically significant by comparison, high concentrations of comparative analysis showed no statistically significant difference while low concentrations of comparative analysis showed statistically significant difference. Conclusion Compared with TPPA method and TP-ELISA method, CMIA method has higher sensitivity and specificity for screening of treponema pallidum antibodies, which helps to improve the accuracy and the positive rate of detection.