超声波辅助提取海膜多糖的工艺优化

Technological optimization of extracting polysaccharide from Halymenia sinesis with ultrasonic-assisted skills

针对海膜藻中可溶性粗多糖的提取工艺进行了初步研究。采用单因素试验和L9(33)正交试验研究了提取时间、提取温度、超声功率、超声时间和料液质量浓度对多糖提取的影响。研究结果表明:最佳水浴提取时间为4 h、提取温度为90℃;料液质量浓度对多糖提取有显著影响,即料液质量浓度是影响多糖提取率的主要因素,其次是超声时间,再次是超声功率;最佳工艺为料液质量浓度0.020 g/mL、超声时间60min、超声功率400 W;多糖提取率为36.91%,总糖含量为63.85%。

This is the first academic report about extracting polysaccharides from Halymenia sinesis （H. sinesis）. H. sinesis and Grateloupia filicina （G. filicina） belong to the same genus, but the study of G. filicina polysaccharides was recorded frequently. With the methods of single factor test and L9 （33） orthogonal experiment, this paper studied how extracting time and extracting temperature affect the extraction of soluble polysaccharide from H. sinesis when using ultrasonic-assisted skills. This paper aims to establish an optimum technique and form the operational procedures of extracting polysaccharides from algae and provide a basis for pilot batches and large-scale production. Experimental results showed that the optimizing of extracting time and extracting temperature were 4 h and 90 ℃, and the concentration of raw material to water （CMW） would significantly affect the extraction percentage of polysaccharides. In addition, CMW was the main factor affecting the extraction percentage of polysaccharide, ultrasonic time was the second factor and ultrasonic power the third. After a set of effective experiments, the extraction yield of polysaccharide could reach 36.91%, and the content of total sugar was up to 63.85% on the conditions of CMW 0. 020 g/mL, ultrasonic time 60 min and ultrasonic power 400 W.