恒定低温下桃小食心虫越冬幼虫体内生化物质变化及耐寒性研究

Study on Cold Tolerance and Variations of Biochemical Substances in Carposina sasakii Matsumura Overwintering Larvae under Constant Low Temperature

为研究低温冷藏对桃小食心虫越冬幼虫耐寒能力的影响,测定了4℃低温冷藏90-210 d期间其过冷却点（SCP）、结冰点（FP）以及甘油、海藻糖和氨基酸含量的变化。结果显示：冷藏时间对桃小食心虫的过冷却点和结冰点影响显著,冷藏90-210 d期间其SCP和FP先升高后降低,第180 d最高（-7.73℃和-4.67℃）,第210 d最低（-13.35℃和-10.58℃）;甘油含量在冷藏第150 d最高（21.37μg/mg）,而此时海藻糖含量最低（21.78μg/mg）;在冷藏期间虫体内均检测到17种氨基酸,冷藏第180 d谷氨酸含量达到最高（3.73 mg/100mg）。

To investigate the effects of cold storage on cold tolerance of Carposina sasakii Matsumura（Lepidoptera：Carposinidae） overwintering larvae,the supercooling point（SCP）,freezing point（FP） and variations of glycerinum,trehalose and amino acid contents were measured during stored at 4℃ for 90 - 210days.The results indicated that cold storage time had significant influences on SCP and FP of Carposina sasakii Matsumura overwintering larvae.The SCP and FP increased firstly and then decreased during storage,and had the highest values（-7.73℃ and-4.67℃） on the 180th day and the lowest values（-13.35℃and-10.58℃） on the 210th day respectively.The content of glycerin was the highest（21.37 μg/mg）while that of trehalose was the lowest（21.78 μg/mg） on the 150th day.Seventeen kinds of amino acids were detected throughout the whole cold storage and the content of Glu reached the maximum（3.73 mg/100mg） on the 180th day.